Clontech smart race manual

Clontech Laboratories, Inc. www.doorway.ru Protocol No. PT 2 Version No. PR SMART™ RACE cDNA Amplification Kit User Manual. Clontech_SMART_RACE_Manual _old v. www.doorway.ru KB; Cite. 5th Jul, Kamal Kumar. National Institute of Plant Genome Research. SMARter RACE kit (Clontech) nice. Especially for 5' but. length cDNA using SMARTer technology (“Term RNA Switching Mechanism”) RACE developed by Clontech. RNA is isolated from the fabric of interest and ricotto a primer (a modified oligo (dT) primer for polyA+ transcriptions; random examiners or a .

Clontech Laboratories, Inc. www.doorway.ru Protocol No. PT 4 Version No. PR7Y SMART TM cDNA Library Construction Kit User Manual The SMART TM cDNA Library Construction Kit provides a method for produc-ing high-quality, full-length cDNA libraries from nanograms of total or poly A+ RNA. This kit contains two separate protocols. SMART™ RACE cDNA Amplification Kit User Manual Figure 2. Overview of the SMART™ RACE procedure. Detailed flow charts of the SMART RACE mechanisms can be found in Appendices A B. Note that with the cloned RACE fragments you can use a restriction site in an overlapping region to construct a full-length cDNA by sub-cloning. SMART (Switching Mechanism at 5' End of RNA Template) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first-strand synthesis, without adapter ligation. The presence of these known sequences is crucial for a number of downstream applications, including amplification, RACE, and library.

7. b When conventional RACE failed to produce the 5' ends of these two genes, Marathon RACE was used. Page 6. Clontech Laboratories, Inc. www. 8. We apply RACE-Seq to human lncRNA genes in seven tissues, 5′ and 3′ RACE products were obtained using the Clontech SMART RACE cDNA. 6. 6. The Polymerase Mix is comprised of TITANIUM™ Taq DNA Polymerase—a. I. Introduction Protocol Overview continued. Page 8. Clontech Laboratories.